Anti-EGFR (phospho-T669) Antibody

A rabbit polyclonal IgG preparation from Antibodies.com is raised against a phosphopeptide surrounding threonine 669 of the human Epidermal Growth Factor Receptor. That residue is phosphorylated by MAPK as part of feedback control over EGFR signaling, making this reagent well suited for studies of receptor down-regulation and ERK-mediated negative feedback. The unconjugated antibody is supplied at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, presented as a 100 µl vial that supports multiple Western blot experiments. The polyclonal format recognizes multiple epitopes around the phospho-T669 region, which can be advantageous when a single monoclonal clone fails in a given lysate preparation. Working aliquots of the glycerol-containing stock should be stored at -20 °C, with limited freeze-thaw cycles to preserve activity. Researchers comparing ligand-stimulated versus basal EGFR phosphorylation, or tracking receptor modulation across time-course and dose-response experiments in human cell models, will find this format a flexible primary antibody choice.