Anti-EGFR (E687) Antibody

EGFR is a 170 kDa transmembrane receptor tyrosine kinase that binds EGF, TGF-α, and related ligands, driving RAS-MAPK and PI3K-AKT signaling in epithelial development and tumor progression. This polyclonal reagent from Antibodies.com was raised in rabbit against an epitope mapping near residue E687 in the cytoplasmic domain of human EGFR, a region containing key autophosphorylation sites that researchers commonly probe when studying receptor activation, dimerization, and internalization.

The antibody is supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline with 15 mM sodium azide at approximately pH 7.2, and is unconjugated for full flexibility with secondary labeling and direct detection chemistries. Validated applications include Western blot, immunohistochemistry, and immunofluorescence, covering fixed-section localization, subcellular trafficking studies, and lysate-based phosphorylation analyses.

Reactivity has been confirmed in human, mouse, and rat samples, making it appropriate for comparative oncology and developmental biology work where rodent models complement human tissue studies. For handling, refrigerate the vial on arrival and aliquot into smaller working volumes to limit freeze-thaw exposure of the sodium azide-preserved solution.