Anti-EGFR Antibody [Assigned #A3598]

This Mouse monoclonal IgG antibody targets the Epidermal Growth Factor Receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase that drives RAS/MAPK and PI3K/AKT signaling cascades upon ligand binding. Supplied by Antibodies.com as a primary antibody in unconjugated format at 100 µl per vial, the reagent is listed for Western blot, immunohistochemistry, and flow cytometry workflows on human samples.

Researchers in cancer biology, signal transduction, and receptor pharmacology use unconjugated EGFR antibodies like this clone to evaluate endogenous receptor levels in carcinoma cell lines, tumor tissue sections, and primary epithelial cultures. The unconjugated format pairs flexibly with secondary antibodies conjugated to HRP, biotin, or fluorophores, supporting both chromogenic IHC detection and multicolor immunofluorescence or flow cytometry panels. As an in vitro research use only reagent, it is suitable for studies of ligand-induced internalization, receptor dimerization, downstream phosphorylation events, and therapeutic resistance in non-small cell lung, colorectal, glioblastoma, and head and neck cancer model systems where EGFR signaling is a known driver.

For handling, store at -20 °C in single-use aliquots to preserve activity across limited freeze-thaw cycles, and centrifuge briefly to bring liquid to the bottom of the vial before opening. The Mouse IgG antibody is reactive with human EGFR and is compatible with standard Western blot band detection at the expected ~170 kDa molecular weight, membrane-localized immunohistochemistry staining, and surface marker analysis by flow cytometry on EGFR-positive cell populations.