Anti-EGFR Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against the epidermal growth factor receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase that activates MAPK and PI3K signaling downstream of EGF and TGF-α binding. The unconjugated reagent is supplied as a 50 µl aliquot and is validated for Western blot and ELISA workflows, supporting readouts of total receptor levels, phosphorylation state, and ligand-driven activation in cell biology and cancer research.

Reactivity has been confirmed against human, mouse, and rat EGFR, allowing the reagent to be used across patient-derived material, murine knockout or transgenic models, and rat tissue lysates within a single experimental program. The polyclonal format increases the chance of recognizing multiple epitopes on the receptor, which is useful when comparing full-length EGFR with the truncated, constitutively active EGFRvIII variant that is characteristic of glioblastoma. Typical applications include monitoring EGFR abundance in tumor lysates, comparing wild-type and mutant receptor expression across cell lines, and tracking changes in receptor levels following small-molecule tyrosine kinase inhibitor treatment.

Because the antibody is unconjugated, it pairs readily with HRP, alkaline phosphatase, or fluorophore-labeled secondary reagents and can be deployed in plate-based immunoassays as either a coating antibody or a detection antibody without adding background from a pre-labeled primary. Storage at -20 °C in single-use aliquots preserves activity across multiple freeze-thaw cycles and limits waste when only small reaction volumes are needed per blot or well. The 50 µl pack size is well matched to pilot studies, antibody screening panels, and shared-instrument core facilities that keep curated inventories of validated primary antibodies.