Anti-EGFR Antibody

Supplied at 100 µl in a vial, this unconjugated rabbit polyclonal antibody from Antibodies.com targets the Epidermal Growth Factor Receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase encoded by ERBB1. The reagent is validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples, where it is used to probe total EGFR expression, study ligand-induced activation of the RAS/MAPK and PI3K/AKT signaling cascades, and characterize receptor overexpression in tumor tissues such as non-small cell lung, colorectal, head and neck, and glioblastoma specimens.

In Western blot applications, the antibody detects the full-length receptor and resolves truncated or mutant variants commonly encountered in research models of tyrosine kinase inhibitor resistance. For immunohistochemistry, it is applied to formalin-fixed paraffin-embedded sections to map receptor distribution across epithelial layers, assess membrane staining intensity in patient biopsies, and correlate expression with downstream pathway activity. The polyclonal format recognizes multiple epitopes on both the extracellular and intracellular domains, supporting detection of wild-type EGFR and a range of clinically relevant mutants. Because the preparation is unconjugated, it pairs flexibly with custom secondary antibodies and reporter systems for both chromogenic and fluorescence-based detection.