Anti-EGFR Antibody

Targeting the epidermal growth factor receptor, this 100 µl polyclonal reagent from Antibodies.com is raised in rabbit and validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows.

The antibody recognizes endogenous EGFR across human, mouse, and rat samples, supporting translational studies that move between cell line models and primary tissue. In western blot applications it produces a clean band at the expected molecular weight for full-length EGFR (~170 kDa), enabling reliable comparison of receptor expression between treated and untreated conditions.

As an unconjugated primary antibody, the reagent can be paired with HRP-conjugated, fluorophore-conjugated, or biotinylated secondary antibodies depending on the detection platform. Common uses include profiling receptor tyrosine kinase signaling in lung, colorectal, breast, and glioblastoma research models, tracking EGFR internalization and recycling in immunofluorescence assays, and confirming expression patterns in patient-derived tissue microarrays.

For storage, keep refrigerated and aliquot upon arrival to minimize freeze-thaw cycles and preserve antibody activity across multiple experiments. This reagent is intended for research use only and is not cleared for diagnostic or therapeutic applications.