Anti-EGFR (Ab-869) Antibody

Raised in rabbit and affinity-purified by antigen-affinity chromatography, this polyclonal reagent from Antibodies.com targets the Epidermal Growth Factor Receptor (EGFR) at the region surrounding tyrosine 869. Supplied as an unconjugated liquid at a working concentration of 1 mg/mL in phosphate-buffered saline (pH 7.4) with 150 mM NaCl, 0.02% sodium azide and 50% glycerol, it is validated for Western blot (recommended dilution 1:500–1:1000) and Immunohistochemistry (1:50–1:100). The antibody is of the rabbit IgG isotype and detects endogenous levels of total EGFR protein in human, mouse and rat samples. The immunogen corresponds to a synthetic peptide spanning residues 867–871 (K-E-Y-H-A) of human EGFR (UniProt P00533), and the expected band migrates at approximately 175 kDa. For stability, the vial should be stored at –20 °C for long-term use and at 4 °C for short-term working aliquots, avoiding repeated freeze-thaw cycles. The 50 µl pack size offered through this Antibodies.com listing is intended for research use only and is not validated for diagnostic or therapeutic applications.