Anti-EGFR (Ab-869) Antibody

Generated against the EGFR kinase domain, this rabbit polyclonal reagent from Antibodies.com recognizes the Epidermal Growth Factor Receptor (EGFR) when phosphorylated at tyrosine 869, a regulatory site tied to receptor activation and downstream signaling through the MAPK and PI3K pathways.

The antibody is supplied as an unconjugated 100 µL vial, raised in rabbit, and validated for Western blot and immunohistochemistry applications. It cross-reacts with EGFR from human, mouse, and rat, supporting comparative studies across tumor-derived cell lines, primary cultures, xenograft samples, and rodent model tissues. The polyclonal format provides multiple epitope recognition, which can be advantageous when probing denatured targets on membranes or fixed sections where accessibility of the phospho-epitope may vary between samples.

Typical uses include tracking EGFR activation status after ligand stimulation (such as EGF or TGF-α treatment), comparing basal versus induced phosphorylation across cell types, and mapping phospho-EGFR distribution in formalin-fixed paraffin-embedded sections. For extended projects, aliquot the antibody to limit repeated freeze-thaw cycles and preserve the phospho-specificity of the reagent.