Anti-EGFR (Ab-1070) Antibody

Targeting the tyrosine 1070 autophosphorylation site within the C-terminal regulatory tail of the epidermal growth factor receptor, this unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum and validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples. Researchers use it to monitor ligand-driven receptor activation, internalization, and downstream signalling through the RAS-MAPK and PI3K-AKT cascades.

Phosphorylation of EGFR at tyrosine 1070 is one of the early autophosphorylation events triggered by EGF binding, making the reagent a marker for receptor activation status rather than total receptor abundance. Investigators in tumour biology, receptor signalling, and cell biology apply it to distinguish quiescent from ligand-stimulated receptor populations in fixed tissue sections and cultured cell lines. The polyclonal format provides broader epitope coverage around the phospho-Y1070 motif, which can improve signal recovery when the target site is partially masked by cross-linking or neighbouring residues.

As a primary antibody, the reagent is compatible with standard chromogenic and fluorescence-based detection systems, and is intended for research use only. It is supplied ready for direct dilution into blocking buffer and is tested for phospho-specificity before release, giving researchers confidence that observed band shifts and staining patterns reflect genuine changes in EGFR activity at this residue rather than variations in overall receptor levels.