Anti-EGFL4 (R1274) Antibody

Antibodies.com offers this rabbit polyclonal reagent targeting EGFL4, a secreted multi-domain protein featuring tandem EGF-like repeats that has been investigated in studies of tissue patterning, cell-matrix interactions, and tumor biology. The antibody was raised against an epitope spanning residue R1274 of the human EGFL4 sequence, providing coverage of the C-terminal region that is useful for researchers examining processed or truncated forms of the protein in human samples.

Validated for Western blot (WB) and immunohistochemistry (IHC), the reagent is supplied as an unconjugated polyclonal antiserum at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, adjusted to approximately pH 7.2. Each vial delivers 100 µL of stock, sufficient for multiple immunoblot membranes or dozens of IHC tissue sections depending on the working dilution selected for each assay. Sodium azide in the storage buffer acts as a preservative; researchers planning downstream conjugation or live-cell staining workflows should account for this additive.

Because EGFL4 expression has been reported in select human tissues and a subset of carcinoma cell lines, this reagent supports laboratories studying developmental gene products, extracellular matrix biology, and oncology biomarker discovery where EGFL4 may serve as a candidate indicator of disease state. The unconjugated format allows flexible pairing with HRP, fluorophore, biotin, or alkaline phosphatase secondary detection reagents according to the chosen experimental protocol.