Anti-EGF Antibody [S-146]

Researchers investigating epithelial cell proliferation, wound healing, and EGFR signaling pathways rely on this mouse IgG1 monoclonal from Antibodies.com, clone S-146, which targets human Epidermal Growth Factor (EGF) and its higher-molecular-weight precursor forms. Supplied as 100 µg of lyophilised, unconjugated antibody, the reagent supports ELISA quantification of growth factor levels, Western blot detection of mature and pro-EGF bands, and immunocytochemistry on cultured cells.

Typical study contexts include salivary growth factor profiling, keratinocyte biology, mucosal repair models, and tumor cell lines in which autocrine EGFR stimulation drives proliferation. The unconjugated format lets users pair the antibody with HRP, biotin, or fluorophore-labelled secondary reagents selected for their preferred detection platform.

After reconstitution in sterile distilled water or suitable buffer, aliquot the antibody to minimise repeated freeze-thaw cycles that erode antigen-binding activity. Store reconstituted vials refrigerated, protect from light when handling at the bench, and reserve a fresh aliquot for each experiment series.