Anti-EF-1 γ Antibody

Elongation Factor 1 gamma (EF-1γ) is a core subunit of the eukaryotic translation elongation complex, where it associates with EF-1α and EF-1β to support protein biosynthesis on the ribosome. Because expression is constitutive across most tissues, this target is widely used as a loading reference alongside β-actin and GAPDH in immunoblot normalization. The Antibodies.com reagent is a rabbit-derived polyclonal IgG preparation supplied unconjugated in a 100 µl volume, validated for Western blot and ELISA detection in human, mouse, and rat sample types. As an affinity-purified polyclonal, the antibody recognizes multiple epitopes on the EEF1G protein, which helps preserve signal when minor denaturation differences arise between samples. Researchers commonly apply this reagent at working dilutions appropriate for chemiluminescent Western blot readouts, where EF-1γ migrates near 50 kDa depending on gel conditions. The unconjugated format offers flexibility to conjugate the antibody in-house with HRP, biotin, or fluorophores, or to use it directly with species-matched secondary reagents. Refrigerated storage preserves activity across repeated use for ongoing experimental programs.