Anti-EEF2 Antibody

Targeting eukaryotic translation elongation factor 2 (EEF2), this rabbit polyclonal primary antibody from Antibodies.com detects the 96 kDa GTPase that catalyzes ribosomal translocation during protein synthesis. Supplied as 100 µl of unconjugated antiserum, the reagent is used for western blot, immunohistochemistry, and immunofluorescence workflows in human, mouse, and rat samples, supporting research in translation control, cancer cell signaling, and neuronal stress responses. EEF2 activity is downregulated by phosphorylation at Thr56 via the kinase EEF2K downstream of mTORC1, so the antibody is widely used to read out elongation status under nutrient deprivation, hypoxia, or chemotherapeutic challenge. The polyclonal format recognizes multiple epitopes on the target, providing flexibility when probing denatured lysates or fixed tissue sections. Pair the antibody with a rabbit-IgG-matched HRP, biotin, or fluorophore secondary for chromogenic DAB staining, fluorescence microscopy, or chemiluminescent detection. Aliquot the working stock and refrigerate to limit freeze-thaw cycles; protect fluorescent conjugates from light during same-day imaging. Research use only.