Anti-EEA1 Antibody

Manufactured by Antibodies.com as a primary antibody reagent for endosomal-trafficking research, this affinity-purified reagent is raised in a rabbit host and is supplied at 1.0 mg/mL in a 50 µL volume. The polyclonal antibody targets the Early Endosome Antigen 1 (EEA1) protein and detects the endogenous level of total EEA1 in human, mouse, and rat samples, having been generated by immunizing rabbits with a synthetic peptide (sequence around amino acids 12–16, R-V-G-S-Q of human EEA1) conjugated to KLH, followed by epitope-specific affinity chromatography. Validated for Western Blot (WB) and supplied unconjugated, the reagent is formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. For handling, the vial is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles minimized to preserve antibody activity. Suitable as an early-endosome marker across standard immunoblotting workflows, the product carries the catalog code A43550 in the Antibodies.com portfolio of primary antibodies.