Anti-EEA1 Antibody

A 100 µl liquid vial of unconjugated rabbit polyclonal antibody to Early Endosome Antigen 1 (EEA1), supplied by Antibodies.com as a Primary Antibody reagent for western blot workflows. The antibody is raised in rabbit against the EEA1 target and detects total endogenous EEA1 protein, making it a reference marker for early endosomes in studies of endocytic trafficking, receptor internalization, and vesicle tethering downstream of Rab5.

Each vial contains 1 mg/ml of IgG in phosphate buffered saline (PBS) at approximately pH 7.2, preserved with 15 mM sodium azide and ready for direct use in standard immunoblotting of whole-cell lysates and tissue homogenates. The polyclonal format recognizes multiple epitopes on the ~170 kDa EEA1 protein, supporting robust signal across the recommended dilution range for chemiluminescent western blot detection.

Verified reactivity covers human, mouse, and rat samples. Researchers use this reagent to probe subcellular fractions enriched for early endosomes and to follow cargo movement from the plasma membrane through the endolysosomal system, including phagosome maturation and autophagy initiation events. Short-term store refrigerated at 4°C; for longer-term storage, aliquot the reagent and freeze to preserve activity across repeated uses.