Anti-ECHS1 Antibody

Enoyl-CoA Hydratase Short Chain 1 (ECHS1) is a mitochondrial matrix enzyme that catalyzes the second step of fatty acid β-oxidation, hydrating trans-2-enoyl-CoA thioesters to L-3-hydroxyacyl-CoA. This unconjugated rabbit polyclonal reagent from Antibodies.com targets the ECHS1 protein for researchers studying mitochondrial energy metabolism, inborn errors of lipid oxidation, and tumor metabolic reprogramming.

The antibody is supplied as 100 µL of polyclonal IgG raised in rabbit, validated for western blot (WB) and immunofluorescence (IF) workflows. It has been tested for cross-reactivity with human, mouse, and rat samples, allowing comparative studies across model systems and patient-derived cell lines. ECHS1 migrates near its expected ~31 kDa molecular weight in reduced SDS-PAGE, supporting clean band identification in mitochondrial lysates.

Typical applications include probing patient fibroblasts for ECHS1 deficiency, mapping the subcellular distribution of the enzyme in tissue sections, and assessing altered expression in cancer cell lines undergoing metabolic switching. For best results, incubate the primary antibody overnight at 4 °C and image immunofluorescence in mitochondria-compatible fixatives such as paraformaldehyde. Store at -20 °C in single-use aliquots to limit freeze-thaw cycles and preserve antibody activity over the shelf life of the reagent. For research use only.