Anti-ECFP Antibody

Enhanced Cyan Fluorescent Protein (ECFP) is a cyan-shifted GFP variant widely adopted as a fluorescent reporter and fusion tag for monitoring gene expression, subcellular protein localization, and protein-protein interactions in transfected cells. This rabbit polyclonal antibody binds ECFP directly and is validated for immunoprecipitation (IP) and western blot (WB) workflows, enabling researchers to confirm expression of ECFP-tagged constructs in cell lysates, verify pull-down efficiency of fluorescent fusion proteins, and detect the fluorophore on immunoblots following SDS-PAGE separation. Distributed by Antibodies.com as a 100 µl volume of unconjugated polyclonal antiserum, the reagent fits standard laboratory protocols that require a primary antibody step before HRP- or fluorophore-conjugated secondary detection, and it complements downstream fluorescence imaging or co-immunoprecipitation experiments in which tagged-protein identity must be confirmed at the biochemical level.