Anti-EBI3 Antibody

Raised in rabbit against the Epstein-Barr virus-induced gene 3 protein, this unconjugated polyclonal reagent from Antibodies.com is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.3. The reagent is validated for Western blot detection of EBI3 in human, mouse, and rat samples, supporting comparative immunology and cytokine-subunit research across species.

EBI3 is a β-subunit that pairs with p28 to form IL-27 and with p35 to form IL-35, two heterodimeric cytokines involved in regulating T helper cell differentiation and regulatory T cell function. Demonstrated cross-reactivity with the human, mouse, and rat orthologues allows comparative expression studies in cell lysates and tissue homogenates prepared under denaturing conditions for SDS-PAGE and subsequent Western blot analysis.

As a polyclonal reagent, the antibody binds multiple epitopes on the target, which can increase signal robustness when EBI3 is present at low abundance. The unconjugated format gives end-users flexibility to pair the antibody with HRP, fluorescent, or biotin-labelled secondary reagents of their choice and to optimize working dilution empirically for each lysate preparation. The product is intended for research use only.