Anti-EBFP Mouse Antibody

A polyclonal antibody raised in mouse hosts against Enhanced Blue Fluorescent Protein (EBFP), supplied unconjugated as 100 µL aliquots in a single vial. EBFP is a widely used fluorescent reporter derived from Aequorea victoria GFP variants, emitting in the blue spectrum with excitation near 383 nm and emission near 447 nm. It is commonly fused to proteins of interest for tracking expression, subcellular localization, and purification in transfected mammalian, bacterial, and insect expression systems.

This reagent from Antibodies.com is validated for Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows. Polyclonal reactivity offers broader epitope coverage than monoclonal alternatives, which is useful when detecting tagged fusion proteins where the EBFP tag may be partially occluded by the fused partner or subject to conformational masking during sample preparation.

The unconjugated format leaves labeling flexible, so end-users can pair the antibody with their preferred secondary antibodies, fluorophores, or enzyme conjugates depending on assay requirements. For research use only. Recommended handling includes aliquoting on receipt to minimize repeated freeze-thaw cycles, and storage at -20 °C for long-term antibody stability. Brief equilibration to room temperature before use helps reduce precipitation.