Anti-EBAG9 Antibody

This unconjugated rabbit polyclonal IgG reagent from Antibodies.com detects EBAG9, a single-pass transmembrane protein also known as RCAS1 (Receptor-binding Cancer Antigen expressed on SiSo cells) or Estrogen receptor-binding fragment-associated gene 9. The target antigen localises to the Golgi membrane, runs at approximately 24 kDa on SDS-PAGE, and acts as a ligand that induces apoptotic cell death in receptor-bearing T lymphocytes, supporting a documented role in tumor immune evasion.

Raised against a recombinant full-length human EBAG9 immunogen and affinity-purified by epitope-specific chromatography, the antibody is supplied as 100 µl of liquid IgG in a single-use vial. It is validated for western blot and immunohistochemistry on human, mouse, and rat samples and is intended for research use only.

In western blot the reagent recognises endogenous EBAG9 in cultured lines such as HEK-293 and HeLa and in rodent heart lysates, producing a band at the expected molecular weight. In IHC it labels formalin-fixed paraffin-embedded sections, with heat-mediated antigen retrieval using Tris-EDTA buffer (pH 9.0) widely adopted in published protocols. Because the antibody is unconjugated, it can be paired in the lab with biotin, HRP, or a fluorophore of choice according to the assay design.

Researchers deploy this reagent to profile EBAG9 in tumor biopsies and cell lines, particularly in breast carcinoma studies that link EBAG9 to estrogen-regulated signalling and to T-cell exclusion from the tumor microenvironment. Published work has also documented EBAG9 release inside cancer-derived extracellular vesicles, making the antibody a useful tool for tracking vesicular EBAG9 transfer in vitro. For short-term storage, refrigerate the vial; for longer-term storage, aliquot the reagent and freeze, avoiding repeated freeze-thaw cycles that can compromise IgG activity.