Anti-EBAG9 Antibody

The EBAG9 protein, also referred to as RCAS1 (receptor-binding cancer antigen expressed on SiSo cells), is a type I transmembrane glycoprotein implicated in tumor immune evasion and the regulation of apoptosis. This unconjugated Rabbit polyclonal reagent from Antibodies.com selectively recognizes EBAG9 in western blot workflows and reacts with human and mouse samples, supporting cross-species study of the antigen in cancer biology, immunology, and cell signaling research.

Produced in rabbit hosts, the antibody is supplied as an IgG preparation at a 100 µl volume, ready for direct incubation with membrane-bound protein lysates. Reducing SDS-PAGE followed by transfer to PVDF or nitrocellulose membranes is the recommended workflow, with signal captured through HRP- or fluorescent-labelled secondary antibodies. The polyclonal nature of this reagent allows recognition of multiple epitopes across the EBAG9 sequence, increasing the likelihood of a robust band even if one site is masked by post-translational modification or sample handling.

Researchers investigating gynecological cancers, breast tumor progression, and T-cell-mediated cytotoxicity pathways commonly apply this reagent, since EBAG9/RCAS1 overexpression has been documented in several malignancies and correlates with reduced patient survival in certain cohorts. Provided in a single-use vial format and shipped under refrigerated conditions to preserve activity, each lot is application-validated and benchmarked against relevant positive-control lysates so investigators can adopt it directly into ongoing western blot protocols.