Anti-E2F6 Antibody

Raised in rabbit against the E2F6 transcription factor, this unconjugated polyclonal antibody from Antibodies.com is supplied as 100 µl of reagent validated for western blot and immunohistochemistry workflows. E2F6 is the atypical member of the E2F family, lacking both the transactivation domain and the pocket-protein binding interface that define its relatives; rather than activating transcription, it assembles into polycomb-type repressor complexes such as PRC1.6 that silence E2F-responsive promoters during S phase, the DNA damage response, and developmental gene programs. Confirmed reactivity against human and mouse samples makes this reagent useful for comparative studies of cell-cycle exit, differentiation, and chromatin-based silencing across these species. The polyclonal format yields broader epitope coverage than a monoclonal reagent, an advantage when probing a protein that interacts with multiple chromatin partners and repressor assemblies. Provided as unconjugated IgG, the antibody is compatible with secondary-detection chemistries of the user's own choice, supporting chromogenic IHC on formalin-fixed tissue sections and chemiluminescent western blot detection of E2F6 in cell and tissue lysates. Aliquot upon receipt and store the preparation as recommended for research-use-only antibodies, avoiding repeated freeze-thaw cycles that can degrade polyclonal IgG preparations.