Anti-E2F6 Antibody

E2F6 is a member of the E2F transcription factor family that acts as a transcriptional repressor during cell cycle progression, particularly at the G1/S boundary, and contributes to DNA damage response and developmental gene silencing through Polycomb-mediated chromatin remodelling. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as a 100 µl vial and is ready for use in standard immunodetection workflows. The antibody has been validated across multiple applications, including western blot analysis of E2F6 protein expression, immunohistochemistry on tissue sections, immunofluorescence for subcellular localisation studies, immunoprecipitation of native E2F6-containing complexes, and chromatin immunoprecipitation (ChIP) for mapping E2F6 occupancy at promoters and enhancers. It cross-reacts with human, mouse, and rat samples, making it suitable for comparative studies in cell line panels, primary cultures, and rodent tissue models. As a polyclonal reagent, it recognises multiple epitopes on the target, which can be advantageous when investigating post-translational modifications or truncated isoforms. Recommended handling includes brief centrifugation before opening and storage in working aliquots to limit freeze-thaw cycles and preserve binding activity.