Anti-E2F4 Antibody

E2F4 contributes to G1/S-phase cell cycle regulation by partnering with pocket proteins such as p130 and p107 within repressive promoter complexes, and the transcription factor has been studied in quiescence, tumor suppression, and developmental biology contexts. The Antibodies.com anti-E2F4 polyclonal antibody is raised in rabbit and supplied at 100 µl per vial as an unconjugated reagent validated for Western blot and immunohistochemistry across human, mouse, and rat samples.

In Western blot, E2F4 is typically resolved as a band near 44 kDa on standard SDS-PAGE gels. For IHC, the reagent has been validated on formalin-fixed paraffin-embedded sections following standard antigen retrieval. The unconjugated format lets the end user pair the antibody with HRP-, biotin-, or fluorophore-conjugated secondaries of their choice. Polyclonal sera generally engage multiple epitopes on the target, which is an advantage with partially degraded samples but a reason to use careful blocking controls, particularly in IHC.

For storage, keep the antibody frozen at -20 °C for long-term stability and aliquot on first use to limit freeze-thaw cycles; short-term handling at 4 °C is appropriate for an active working aliquot. The reagent is intended for research use only.