Anti-E2F1 (phospho-T433) Antibody

The Antibodies.com polyclonal antibody targets the phosphorylated form of the E2F1 transcription factor at threonine 433, a post-translational modification tied to cell-cycle progression and apoptotic signaling pathways. Raised in rabbit, this unconjugated reagent is supplied at 1 mg/ml in phosphate buffered saline (pH 7.2) containing 0.05% sodium azide as a preservative, and is validated for immunohistochemistry on fixed tissue sections. The antibody recognizes both human and mouse E2F1 when phosphorylated at T433, supporting studies in oncology and cell-cycle biology where site-specific phosphorylation status is being mapped across patient samples or model organisms.

Each vial contains 100 µl of antibody, suitable for multiple staining runs depending on dilution. Because the reagent is phospho-specific, researchers frequently pair it with total E2F1 antibodies to compare phosphorylated versus baseline levels, and with HRP-conjugated secondary antibodies for chromogenic IHC detection. Refrigerate on receipt and protect from light to preserve activity; aliquot to avoid repeated freeze-thaw cycles.