Anti-E2F1 Antibody [Assigned #A2442]

A primary mouse monoclonal IgG reagent raised against recombinant human E2F1 protein and affinity purified for batch-to-batch consistency. The antibody is supplied unconjugated as a 50 µl liquid aliquot, ready to be paired with the secondary detection system of the researcher's choice for western blot, immunofluorescence, and immunoprecipitation workflows.

Clone #A2442 recognises an epitope on the ~70 kDa E2F1 transcription factor and has been verified to cross-react with human, mouse, and rat samples. Recommended working dilutions are 1:200 to 1:1000 for western blot, 1:50 to 1:100 for immunofluorescence, and 1:20 to 1:50 for immunoprecipitation, giving flexibility across routine immunoblotting, fixed-cell imaging, and native complex pull-down assays in research use only settings.

Upon arrival, refrigerate the vial, protect from light, and aliquot if repeated use is planned to limit freeze-thaw cycles. The unconjugated format keeps the reagent compatible with custom HRP, fluorophore, or biotin labelling schemes used in cell cycle, DNA damage response, and retinoblastoma pathway studies.

Antibodies.com distributes this E2F1 reagent as a practical addition to proliferation-marker and tumor suppressor network panels.