Anti-E2F1 Antibody [Assigned #A2442]

This Antibodies.com primary antibody targets E2F1, a transcription factor central to cell cycle regulation and the G1/S transition. E2F1 governs DNA replication, apoptosis, and tumor suppression pathways, making it a focus in cancer biology, proliferation studies, and gene expression analyses.

The reagent is a mouse monoclonal IgG antibody (clone A2442) supplied as an unconjugated preparation in a 200 µl vial volume. Reactivity has been characterized against human, mouse, and rat samples, supporting cross-species investigations in cell line and primary tissue models.

Validated applications include western blot for expression profiling, immunofluorescence for subcellular localization, and immunoprecipitation for mapping protein-protein interactions. Researchers working on pRB pathway dynamics, oncogenic signaling, or chemotherapy response will find this reagent adaptable to both in vitro cell-based assays and tissue-based studies. Aliquot upon receipt and store at -20°C in working volumes to limit freeze-thaw degradation.