Anti-E2F1 Antibody

From Antibodies.com, this is a rabbit polyclonal antibody raised against E2F1. The unconjugated IgG reagent is validated for Western blot (WB) and immunofluorescence applications (IF / ICC/IF), and it reacts with Human, Mouse and Rat samples.

The antibody was generated using a recombinant fusion protein corresponding to amino acids 301–437 of human E2F1 (UniProt Q01094). It is affinity-purified and supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.01% thiomersal. The reported molecular weight for the target is 70 kDa. Recommended starting dilutions are 1:500–1:1,000 for WB and 1:20–1:100 for ICC/IF.

The product is shipped at 4°C; on receipt it should be aliquoted and stored at –20°C, with freeze/thaw cycles avoided.