Anti-E2F1 Antibody

Antibodies.com provides this rabbit polyclonal reagent raised against human E2F1, a transcription factor that governs the G1 to S phase transition and couples DNA damage signaling to apoptotic outcomes. Supplied as an unconjugated preparation in a 100 µl vial, the antibody recognizes the native target in human samples and is validated for Western blot (WB) and immunofluorescence (IF) workflows.

The 100 µl volume supports multiple immunoblot membranes or IF staining runs. Investigators studying retinoblastoma pathway regulation, p53-mediated apoptosis, or oncogenic transformation routinely probe E2F1 protein levels in cultured cell lines and tumor-derived lysates. Because the target participates in checkpoint control rather than constitutive housekeeping, treated-versus-untreated comparisons tend to deliver the most informative readouts. The unconjugated format also leaves the antibody compatible with secondary reagents of the researcher's choice, including HRP, Alexa Fluor, or biotin conjugates.

Aliquoting upon first thaw and refrigeration between uses preserves activity across the working shelf life; because the reagent is unconjugated, light-sensitive handling is not required.