Anti-E2F1 Antibody

E2F1 (E2F Transcription Factor 1) is a member of the E2F family of transcription factors that orchestrates the transition from G1 to S phase of the cell cycle by regulating genes required for DNA replication and cell proliferation. This rabbit polyclonal antibody recognizes human E2F1 and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. The unconjugated format gives researchers flexibility to build custom detection schemes, including indirect immunolabeling and signal amplification strategies when target abundance is low.

Produced in rabbit hosts, the polyclonal formulation provides recognition of multiple epitopes on E2F1, which improves tolerance for minor sample variation and reduces sensitivity to single-residue changes. Each vial is supplied as 100 µl of reagent, sufficient for several immunoblot membranes or a series of stained tissue sections.

From Antibodies.com, this reagent is targeted specifically to the human E2F1 protein and is widely deployed in studies of cell proliferation, tumorigenesis, and the Rb/E2F regulatory axis that is frequently disrupted in cancer. Researchers working on apoptosis, the DNA damage response, or transcriptional control mechanisms often include E2F1 readouts in their experimental pipelines, and this antibody supports both qualitative localization in fixed tissues and quantitative expression analysis by immunoblotting.