Anti-E Cadherin Antibody

This polyclonal rabbit antibody, offered by Antibodies.com in a 100 µg liquid vial format, specifically recognizes E-cadherin (also known as CDH1 or Cadherin-1), the prototypical calcium-dependent adhesion molecule of the classical cadherin superfamily. E-cadherin mediates homotypic cell-cell adhesion within epithelial tissues, sustaining adherens junction integrity through its cytoplasmic linkage to β-catenin and the actin cytoskeleton, and its loss is a recognized hallmark of epithelial-mesenchymal transition (EMT) during carcinoma invasion.

Validated for western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry (ICC), and flow cytometry (FC), this reagent reacts with human and mouse E-cadherin, supporting research in epithelial polarity, tumor metastasis, and developmental biology. Aliquot upon receipt and store at -20 °C in a frost-free freezer; avoid repeated freeze-thaw cycles to preserve antibody titer and binding activity.