Anti-Dynactin 1 (N-terminus) Antibody

Offered by Antibodies.com as a research-grade primary antibody, this reagent is a mouse IgG2b monoclonal that targets the N-terminus of Dynactin 1 (DCTN1), the 150 kDa dynein-associated polypeptide also known as p150^Glued. The antibody was raised against purified recombinant human DCTN1 protein fragments expressed in E. coli and is affinity purified before being supplied as a liquid formulation in 0.1 M Tris-Glycine buffer (pH 7.4) containing 150 mM NaCl, 0.2% sodium azide, and 50% glycerol; it should be stored according to the supplier's recommendations (typically –20 °C in aliquots to limit freeze–thaw cycles). The product is unconjugated and is validated for multiple applications, including Western blotting (WB), ELISA (E), and immunocytochemistry (ICC), with the supplier demonstrating clean detection of the predicted 150 kDa band in A2780, TF-1, HepG2, MCF7, A431, and K562 cell lysates at a 1:1000 dilution. The host species is mouse, the clonality is monoclonal, the isotype is IgG2b, and the antibody is reported to cross-react with Human, Mouse, and Bovine samples, making it suitable for cytoskeletal and intracellular transport studies in a range of model systems. Each vial is intended for research use only and is not for diagnostic or therapeutic applications.