Anti-DYKDDDDK Tag Antibody [Flag.C2]

For laboratories working with FLAG-tagged recombinant proteins expressed in human cells, this unconjugated mouse monoclonal IgG1 reagent from Antibodies.com provides a well-characterised detection tool. The clone, Flag.C2, binds the eight-amino-acid DYKDDDDK epitope (commonly called the FLAG tag) with high specificity and is supplied as a lyophilised powder in a 500 µg aliquot.

Validated applications include Western blotting, immunoprecipitation, and immunostaining of fixed samples, covering the full workflow from confirming expression of a tagged construct through to pulling down the fusion partner and visualising its localisation. The unconjugated format leaves the choice of secondary reporter (HRP, fluorophore, biotin, or bead conjugate) to the end user, which makes the reagent a flexible building block for custom assay panels.

Reconstitution is straightforward in PBS or similar buffer; lyophilised storage eliminates the cold-chain requirement during shipping. The IgG1 isotype is compatible with standard anti-mouse secondary reagents, and the antibody is widely cited in published studies of N-terminal, C-terminal, and internal FLAG fusions used in recombinant protein production and affinity purification workflows.