Anti-DYKDDDDK Tag Antibody

The Anti-DYKDDDDK Tag Antibody from Antibodies.com is a mouse monoclonal IgG1 reagent that recognizes the hydrophilic FLAG octapeptide, a widely engineered fusion tag appended to recombinant proteins for affinity purification and detection workflows. Supplied as a liquid at 100 µg per vial and validated for Western Blot, Immunofluorescence, and Immunoprecipitation, it binds FLAG-tagged fusion proteins expressed in mammalian, insect, and bacterial lysates without significant cross-reactivity against common His, HA, or Myc epitope tags. Aliquot upon arrival, store refrigerated for short term use, and for longer storage keep frozen at -20°C while avoiding repeated freeze-thaw cycles. The unconjugated format provides flexibility for pairing with secondary reporters of choice, and the antibody performs reliably in co-immunoprecipitation experiments to pull down FLAG-tagged baits together with their interacting partners.