Anti-DUT Antibody

Deoxyuridine triphosphatase (dUTPase), encoded by the DUT gene, hydrolyzes dUTP to dUMP and pyrophosphate, a reaction that prevents misincorporation of uracil into replicating DNA and channels dUMP toward thymidylate biosynthesis. This rabbit polyclonal anti-DUT antibody from Antibodies.com was raised against the human DUT target and recognizes a conserved epitope shared across human, mouse, and rat orthologs, supporting cross-species experimental design. The unconjugated IgG preparation is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, shipped as a 100 µl aliquot suitable for multiple immunoblotting or staining experiments. Validated applications include Western blot for detection of dUTPase expression in cell lysates and tissue extracts, and immunohistochemistry (IHC) for localization studies in formalin-fixed paraffin-embedded sections. The polyclonal format improves tolerance for minor sequence variation between species and is commonly used to probe dUTPase upregulation in tumor samples, thymidylate synthesis pathway studies, and DNA replication fidelity research. Storage at -20 °C in the provided glycerol buffer preserves activity across freeze-thaw cycles limited to short-term use.