Anti-DUSP8 Antibody

The Anti-DUSP8 polyclonal antibody offered by Antibodies.com is a rabbit-derived IgG reagent raised against Dual Specificity Phosphatase 8, a dual-specificity phosphatase that dephosphorylates phosphotyrosine and phosphoserine/phosphothreonine residues on stress-activated MAP kinases including JNK and p38 family members. Provided as an unconjugated 100 µl liquid aliquot, this primary antibody supports Western blot detection of the endogenous human DUSP8 protein in cell lysate and tissue homogenate samples.

Each preparation is generated in rabbit and purified to yield a polyclonal IgG reagent that recognizes epitopes on the human DUSP8 target. The reagent ships unconjugated, allowing researchers to couple it directly to HRP, biotin, or a fluorophore of their choice when configuring custom Western blot workflows. Because DUSP8 is implicated in regulation of the JNK signaling cascade, oxidative stress responses, and metabolic pathways, this antibody is a useful tool for studies examining signal transduction, cellular stress biology, and kinase pathway dephosphorylation in human cell lines and primary tissue samples.

Recommended use is Western blot, with sample preparation typically involving RIPA-based lysis, SDS-PAGE separation, and transfer to nitrocellulose or PVDF membranes. Keep the antibody refrigerated between uses and aliquot upon arrival to preserve activity across multiple experiments. Researchers working with non-human species should validate cross-reactivity independently, as reactivity is confirmed for the human target.