Anti-Drosha Antibody

Generated in rabbit against the Drosha ribonuclease III enzyme, this polyclonal antibody from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, adjusted to approximately pH 7.2. The unconjugated reagent is validated for western blot detection of endogenous Drosha in human, mouse, and rat samples.

Drosha forms the catalytic core of the nuclear Microprocessor complex, where it partners with the double-stranded RNA-binding protein DGCR8 to crop primary microRNA transcripts into precursor hairpins during the first step of miRNA biogenesis. It also participates in the processing of other non-coding RNA substrates, making it a focus of research in gene regulation, stem-cell biology, and pathologies linked to dysregulated microRNA expression.

The reagent arrives as a 100 µl volume that supports multiple immunoblotting experiments. Aliquot upon receipt and store at -20°C, avoiding repeated freeze-thaw cycles to preserve activity. The polyclonal format provides broader epitope coverage than monoclonal alternatives, which is useful when probing full-length Drosha across tissues and species.