Anti-DNMT3L Antibody

Raised in rabbit against the human DNMT3L target, this polyclonal reagent from Antibodies.com is supplied as an unconjugated, ready-to-use IgG preparation. Each vial contains 50 µl of antibody at a concentration of 1 mg/ml, suitable for sensitive detection in routine chromatin and methylation studies. The antibody has been validated for Western blot (WB) and immunohistochemistry (IHC) workflows, and is reactive with human, mouse and rat samples, giving broad cross-species utility for epigenetics and developmental biology research. Because the reagent is unconjugated, it is fully compatible with secondary-labeling strategies such as HRP, fluorescent or biotin detection, allowing researchers to tailor the assay to their preferred platform. The host species (rabbit) and polyclonal clonality make this reagent a strong choice for recognising multiple epitopes on the DNMT3L protein, which can be particularly valuable when studying the catalytically inactive DNA methyltransferase cofactor. For long-term stability and reproducible performance, the manufacturer recommends continuous storage at –20 °C, avoiding repeated freeze-thaw cycles by aliquoting on first use. Suitable applications include western blotting of DNMT3L expression in cell lysates and immunostaining of tissue sections to localise the protein in germ cells and early embryonic contexts. This Antibodies.com primary antibody offers a reliable, research-grade option for laboratories investigating DNA methylation patterning, genomic imprinting and the supporting role of DNMT3L in de novo methyltransferase activity alongside DNMT3A and DNMT3B.