Anti-DNMT3B Antibody

Raised in rabbits and supplied as an unconjugated polyclonal IgG, this Primary Antibody reagent from Antibodies.com recognizes DNMT3B, the de novo DNA methyltransferase that establishes CpG methylation patterns during early embryogenesis and in somatic tissues. The reagent detects endogenous DNMT3B protein in human, mouse, and rat samples through Western blot, immunohistochemistry, and immunofluorescence workflows, with the polyclonal format enabling recognition of multiple epitopes across the full-length target.

Each vial contains 200 µl of antibody in a phosphate-buffered formulation preserved with sodium azide. Aliquot on receipt and store frozen at -20°C to preserve activity across repeated freeze-thaw cycles; protect the reagent from light during extended storage. The unconjugated format allows flexible pairing with HRP, fluorophore, or biotin conjugates of the researcher's choice for downstream immunodetection, chromogenic imaging, or signal amplification strategies.

Use cases in epigenetic research include mapping DNMT3B expression across tissue sections, confirming protein-level changes following CRISPR or RNAi manipulation of methyltransferase pathways, and characterizing chromatin-associated fractions. In disease models, the reagent supports studies of ICF syndrome type 1, in which loss-of-function mutations disrupt centromeric satellite repeat methylation, as well as oncology investigations of DNMT3B overexpression in lung, breast, and colorectal tumor systems. X-chromosome inactivation, genomic imprinting, and somatic methylome reprogramming studies also benefit from robust detection of the endogenous protein across the supported species.