Anti-DNMT3A Antibody

DNA methyltransferase 3A is the de novo methyltransferase that establishes CpG methylation patterns during mammalian development and adult hematopoiesis, and loss-of-function mutations in this chromatin regulator are recurrent drivers of acute myeloid leukemia. This rabbit polyclonal reagent from Antibodies.com binds DNMT3A and is validated for Western blot and immunofluorescence detection in human samples, supplied unconjugated at 100 µl. Researchers investigating epigenetic reprogramming, X-chromosome inactivation, genomic imprinting, or cancer methylome remodeling apply the antibody to nuclear extracts or fixed cells, where it produces a nuclear staining pattern consistent with the enzyme's tight association with chromatin and pericentromeric repeats. The polyclonal format recognizes multiple epitopes across the protein, supporting detection of full-length DNMT3A together with several shorter isoforms that have been reported in lymphoid and myeloid lineages. For Western blot, the reagent is compatible with standard PVDF or nitrocellulose transfer and chemiluminescent read-out using anti-rabbit IgG secondary antibodies; for immunofluorescence, it is suited to methanol- or paraformaldehyde-fixed cell preparations counterstained with DAPI. The vial is intended for several short-term experiments when kept refrigerated and protected from light between uses, helping preserve activity across a typical research project. The unconjugated format also allows end-users to conjugate the antibody themselves to fluorophores, biotin, or HRP if a custom detection chemistry is required. For research use only.