Anti-Dnmt1 Antibody

A rabbit polyclonal primary antibody targeting Dnmt1 (DNA (cytosine-5)-methyltransferase 1), the maintenance methyltransferase responsible for copying CpG methylation patterns to daughter DNA strands during replication. This unconjugated reagent from Antibodies.com is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as a 50 µl vial that can be used directly or conjugated as needed.

Validated across several immunoassays, the antibody is used in western blot detection of the ~183 kDa Dnmt1 protein, immunohistochemistry on fixed tissue sections, immunofluorescence imaging of nuclear staining, immunoprecipitation of endogenous Dnmt1-containing complexes, and chromatin immunoprecipitation (ChIP) for mapping methylated genomic loci. Reactivity has been confirmed in human, mouse, and rat samples, enabling cross-species epigenetics studies.

In the lab, researchers apply this reagent to studies of X-chromosome inactivation, genomic imprinting, embryonic development, and cancer epigenetics. The glycerol-stabilized formulation should be stored frozen at -20°C and aliquoted to minimize freeze-thaw cycles and preserve antibody activity across repeated experiments.