Anti-DNase I Antibody

Antibodies.com supplies this rabbit polyclonal reagent raised against human DNase I, the ~31 kDa endonuclease that hydrolyzes phosphodiester bonds in single- and double-stranded DNA to release 5'-phosphorylated oligonucleotides. Provided as an unconjugated 100 µl aliquot, the antibody is validated for western blot and immunofluorescence workflows, supporting detection of the enzyme in cell lysates, frozen tissue sections, and fixed cellular samples. The polyclonal format recognizes multiple epitopes on the target, which improves signal recovery when examining truncated fragments produced during apoptosis or neutrophil extracellular trap release. Because the reagent is unconjugated, users can pair it with a chosen secondary antibody, fluorophore, or enzymatic reporter to match their specific imaging or blotting setup. Altered DNase I activity has been associated with systemic lupus erythematosus, vascular injury, and tumor microenvironment remodeling, making this antibody relevant for immunology, cell biology, and apoptosis studies where altered nuclease behavior is the focus.