Anti-DNA pol α Antibody

DNA polymerase alpha (DNA pol α) is the four-subunit enzyme that primes eukaryotic DNA replication, and this rabbit polyclonal antibody is raised against the human protein for detection in Western blot and ELISA workflows. Antibodies.com supplies the reagent as an unconjugated 100 µl aliquot of polyclonal antiserum, ready to pair with a standard anti-rabbit secondary reagent during immunodetection.

The catalytic subunit p180 carries the polymerase activity, while the associated primase subunits generate the short RNA primer that is later extended by Pol δ and Pol ε at replication forks. Researchers working on origin firing, S-phase entry, replication stress, and chromatin-bound replisome dynamics use this reagent to monitor protein levels in whole-cell lysates from human lines such as HeLa, HEK293, and U2OS. In immunoblots it yields a band consistent with the catalytic subunit, and the human reactivity profile listed by Antibodies.com makes it useful for studies using patient-derived cells and standard human culture systems.

For Western blot, dilute the primary in blocking buffer according to the lot-specific datasheet and detect with HRP- or fluorescence-labelled anti-rabbit IgG. Aliquot the 100 µl vial on first use, store refrigerated at 4°C for short-term working stocks, and freeze at -20°C for longer term; limiting freeze-thaw cycles preserves antibody activity. Intended for research use only, not for diagnostic or therapeutic applications.