Anti-DNA pol ε 2 Antibody

Raised in rabbit against DNA polymerase epsilon subunit 2 (POLE2), this unconjugated polyclonal reagent from Antibodies.com is supplied as a 100 µl vial at 1 mg/ml in PBS with 15 mM sodium azide at approximately pH 7.2. Validated for Western blot detection in human, mouse, and rat samples, the antibody recognizes the accessory subunit of the leading-strand polymerase complex and is used to study replication fork stability, S-phase checkpoint signaling, and chromatin-associated DNA repair. Laboratories investigating genome integrity, replisome assembly, and CMG helicase loading apply this reagent to cell-line lysates, xenograft extracts, and primary tissue homogenates. Working aliquots should be refrigerated and the stock stored frozen at -20 °C; limit repeated freeze-thaw cycles by preparing single-use portions and avoid additional sodium azide before downstream coupling chemistries.