Anti-DNA-PKcs Antibody

The Antibodies.com Anti-DNA-PKcs monoclonal antibody targets the catalytic subunit of DNA-dependent protein kinase, a ~470 kDa serine/threonine kinase central to the DNA damage response and non-homologous end joining (NHEJ) repair pathway. Raised in mouse hosts and supplied as an IgG2b isotype, this unconjugated reagent is validated for Western blot (WB), immunoprecipitation (IP), and immunocytochemistry (ICC) workflows against human samples. DNA-PKcs phosphorylates substrates including p53 and c-Myc following double-strand break induction, supporting research into genomic stability, V(D)J recombination, radiosensitivity, telomere maintenance, and cancer biology. Storage at 4 degrees C is recommended for short-term use, with aliquoting advised to limit freeze-thaw cycles and preserve antibody activity across multiple experiments. The 100 µl vial format provides sufficient reagent for several replicate assays, and the unconjugated state allows flexible pairing with user-selected secondary antibodies or custom labeling strategies for downstream detection.