Anti-DNA-PKCS Antibody

Sourced from Antibodies.com, this primary antibody is a purified mouse monoclonal raised against DNA-PKCS (DNA-dependent protein kinase catalytic subunit), the catalytic core of the DNA-PK complex involved in non-homologous end joining and V(D)J recombination. The reagent is supplied at 50 µl in an unconjugated format, presented as a purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide and 50% glycerol, and is of the IgG2b isotype. It is validated for Western Blotting (WB), Immunoprecipitation (IP) and Immunocytochemistry (ICC/IF) workflows, and has been confirmed to react with human samples, where it detects endogenous levels of the ~450 kDa DNA-PKcs protein encoded by the PRKDC gene. Researchers are advised to store the vial at –20 °C for long-term stability (with short-term handling at 2–8 °C) and to avoid repeated freeze-thaw cycles to preserve antibody activity. Provided through the Antibodies.com catalogue as a Research Use Only reagent, this clone is intended for laboratory applications studying DNA damage response, double-strand break repair, and related signalling pathways in human cell models.