Anti-DNA-PKCS Antibody

This Mouse monoclonal antibody targets the catalytic subunit of DNA-dependent protein kinase (DNA-PKCS, encoded by PRKDC), a ~470 kDa serine/threonine kinase essential for non-homologous end joining (NHEJ) repair of DNA double-strand breaks, V(D)J recombination, and telomere maintenance. The unconjugated reagent is supplied by Antibodies.com as 100 µL of purified IgG in 0.1 M Tris-Glycine buffer (pH 7.4) with 150 mM NaCl, 0.2% sodium azide as preservative, and 50% glycerol for storage at -20 °C. Validated for western blot, immunoprecipitation, and immunocytochemistry on human samples, the antibody recognizes the endogenous full-length protein and is commonly used to study ATM-independent DNA damage response signaling, p53 phosphorylation events, and radiosensitivity phenotypes in cell culture and in vitro models. For research use only; aliquot upon receipt to limit freeze-thaw cycles and protect from light.