Anti-DNA-PK Antibody

Target the catalytic subunit of DNA-dependent protein kinase with this Rabbit polyclonal antibody raised against DNA-PK (DNA-PKcs), a serine/threonine kinase central to the DNA damage response, non-homologous end joining, and V(D)J recombination. Antibodies.com supplies this unconjugated primary antibody in a 50 µl vial, validated for Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) on human samples.

Laboratories studying double-strand break repair, cellular radiosensitivity, genomic stability, or tumour biology use this reagent to monitor total DNA-PKcs expression, autophosphorylation events following DNA damage, or nuclear localisation of the kinase complex. The polyclonal format recognises multiple epitopes across the large catalytic polypeptide, supporting reproducible detection across blotting membranes and fixed tissue sections. Host species is Rabbit and reactivity is reported as Human, making it suitable for work on patient-derived cell lines, primary cultures, and archived clinical specimens.

For best results, store at -20 °C in working aliquots and limit freeze-thaw cycles to preserve antibody activity. The unconjugated format allows pairing with anti-rabbit HRP secondary reagents for chromogenic Western blots, or with fluorophore-conjugated secondaries for fluorescent imaging and immunofluorescence microscopy. Supplied as a research-use-only reagent and intended for non-diagnostic laboratory applications.