Anti-DNA ligase 3 Antibody

This polyclonal antibody from Antibodies.com targets human DNA ligase 3 (LIG3), an ATP-dependent enzyme that seals single-strand DNA breaks during base excision repair and other DNA damage response pathways. Raised in rabbit and supplied unconjugated in a 100 µl volume, the reagent is validated for Western blot (WB) and immunohistochemistry (IHC) workflows and reacts with human samples, supporting studies of genomic stability, nucleotide repair mechanisms, and the broader DNA damage response.

DNA ligase 3 functions within an XRCC1 complex at sites of single-strand break repair and contributes to mitochondrial DNA maintenance through an alternative translation product. Researchers working on cancer biology, neurodegeneration, or DNA repair-deficient disease models rely on consistent LIG3 detection to monitor repair capacity, map subcellular localization in fixed tissues, and confirm expression changes following genotoxic stress or therapeutic intervention. The polyclonal format yields recognition of multiple epitopes across the target, useful when probing full-length LIG3 and its known isoforms.

For Western blot, the antibody is applied to whole-cell or tissue lysates to detect endogenous LIG3 expression. For IHC, it supports chromogenic or fluorescent staining of formalin-fixed paraffin-embedded sections to visualize nuclear and mitochondrial distribution patterns in tissue architecture. Aliquot on first use and avoid repeated freeze-thaw cycles to preserve activity across multiple experiments.