Anti-Dkk-3 Antibody

Antibodies.com offers this rabbit-derived polyclonal reagent for detecting Dkk-3 (Dickkopf-related protein 3), a secreted modulator of Wnt signaling that has been investigated in tumor biology and renal pathophysiology. The unconjugated antibody is supplied as 100 µl of liquid stock and is validated for Western blot and immunofluorescence workflows on human samples and cell lines.

The reagent recognizes endogenous human Dkk-3 and is suitable for studies of canonical and non-canonical Wnt pathway regulation, where altered Dkk-3 expression has been linked to glioma, renal injury, and several carcinomas. As a polyclonal reagent raised in rabbit, it provides broad epitope coverage, which is helpful when probing truncated or post-translationally modified forms of the protein in lysate or fixed-cell preparations. Standard blotting protocols on whole-cell or tissue extracts typically yield a clear band in the expected molecular weight range.

Storage and handling: brief centrifugation prior to opening recovers the full volume, and aliquoting upon first use minimizes freeze-thaw cycles that can reduce binding activity over time. The antibody is provided without a fluorophore or enzyme conjugate, allowing researchers to pair it with their own secondary detection reagents for fluorescent, chromogenic, or chemiluminescent readouts depending on the chosen application.